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KMID : 1007520090180040959
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Food Science and Biotechnology
2009 Volume.18 No. 4 p.959 ~ p.964
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Optimization of Medium Composition for Production of the Antioxidant Substances by Bacillus polyfermenticus SCD Using Response Surface Methodology
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Lee Jang-Hyun
Chae Mi-Seung
Choi Gooi-Hun
Lee Na-Kyoung
Paik Hyun-Dong
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Abstract
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Production of the antioxidant substances by Bacillus polyfermenticus SCD was investigated using shake-flaskfermentation. The one-factor-at-a-time method was first employed to determine the key ingredients for optimal mediumcomposition, then further investigation of the medium composition was performed using response surface methodology(RSM). The antioxidant activity was measured using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assays. After screeningvarious elements, fructose, tryptone, and MgSO4·7H2O were chosen as the main factors for study in the statisticalexperimental design. Central composite design (CCD) was then used to determine the optimal concentrations of these 3components. Under the proposed optimized medium containing 2.8% fructose, 1.34% tryptone, 0.015% MgSO4·7H2O, 0.5%NaCl, and 0.25% K2HPO4, the model predicted an antioxidant activity of 80.5% (R2=0.9421). The actual experimental resultswere in agreement with the prediction.
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KEYWORD
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Bacillus polyfermenticus SCD, antioxidant substance, medium optimization, central composite design, responsesurface methodology
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